Publications

2025

Domain coupling in activation of a family C GPCR. Latorraca NR, Sabaat S, Habrian CH, Bleier J, Stanley C, Kinz-Thompson CD, DMarqusee S, Isacoff EY. Nat Chem Biol. 2025 Apr 25. doi: 10.1038/s41589-025-01895-3.  PMID: 40281344

Dopamine D1 receptor activation in the striatum is sufficient to drive reinforcement of anteceding cortical patterns. Vendrell-Llopis N, Read J, Boggiano S, Hetzler B, Peitsinis Z, Stanley C, Visel M, Trauner D, Donthamsetti P, Carmena J, Lammel S, Isacoff EY. Neuron. 2025 Mar 5;113(5):785-794.e9. doi: 10.1016/j.neuron.2024.12.013. Epub 2025 Jan 14. PMID: 39814009

Behavioral resilience via dynamic circuit firing homeostasis. Hoagland A, Schultz R, Cai Z, Newman ZL, Isacoff EY. Proc Natl Acad Sci U S A. 2025 May 6;122(18):e2421386122. doi: 10.1073/pnas.2421386122. Epub 2025 Apr 29. PMID: 40299703

Deficiency in transmitter release triggers homeostatic transcriptional changes that increase presynaptic excitability. Cypranowska CA, Feldthouse M, Choi YG, Bakshinska D, Li R, Newman ZL, Isacoff EY. Proc Natl Acad Sci USA. 2025 Aug 5;122(31):e2322714122. doi: 10.1073/pnas.2322714122. Epub 2025 Jul 29. PMID: 40729383; PMCID: PMC12337328.

Synapse-specific catecholaminergic modulation of neuronal glutamate release. Bakshinska D, Liu WY, Schultz R, Stowers RS, Hoagland A, Cypranowska C, Stanley C, Younger SH, Newman ZL, Isacoff EY. Proc Natl Acad Sci U S A. 2025 Jan 7;122(1):e2420496121. doi: 10.1073/pnas.2420496121. Epub 2024 Dec 30. PMID: 39793084

Balanced synapse-to-synapse short-term plasticity ensures constant transmitter release. Aghi K, Schultz R, Stowers RS, Liu WY, Mendonça PRF, Li R, Bakshinska D, Newman ZL, Isacoff EY. Curr Biol. 2025 Jun 23;35(12):2881-2892.e6. doi: 10.1016/j.cub.2025.05.024. Epub 2025 Jun 3. PMID: 40466645; PMCID: PMC12439520.

Structure and dynamics determine G protein coupling specificity at a class A GPCR. Casiraghi M, Wang H, Brennan PC, Habrian C, Hübner H, Schmidt MF, Maul L, Pani B, Bahriz SMFM, Xu B, Staffen N, Assafa TE, Chen B, White E, Sunahara RK, Inoue A, Xiang YK, Lefkowitz RJ, Isacoff EY, Nucci N, Gmeiner P, Lerch MT, Kobilka BK. Sci Adv. 2025 Mar 21;11(12):eadq3971. doi: 10.1126/sciadv.adq3971.  PMID: 40106559

Reversible Antagonism of Dopamine D1 Receptor using a Photoswitchable Remotely Tethered Ligand.  Hetzler, B.E., Donthamsetti, P., Wolesensky, R.M., Stanley, C., Isacoff, E.Y. and Trauner, D. (2025). ACS Chemical Biology  2025 Nov 21;20(11):2609-2616. doi: 10.1021/acschembio.5c00441. PMID: 41099437

2024

Subtype-specific conformational landscape of NMDA receptor gating. Bleier J, Furtado de Mendonca PR, Habrian CH, Stanley C, Vyklicky V, Isacoff EY. Cell Rep. 2024 Aug 27;43(8):114634. doi: 10.1016/j.celrep.2024.114634. Epub 2024 Aug 17. PMID: 39154344 

Coupling sensor to enzyme in the voltage sensing phosphatase. Yu Y, Zhang L, Li B, Fu Z, Brohawn SG, Isacoff EY. Nat Commun. 2024 Jul 30;15(1):6409. doi: 10.1038/s41467-024-50319-8. PMID: 39080263 

Stepwise activation of a metabotropic glutamate receptor. Krishna Kumar K, Wang H, Habrian C, Latorraca NR, Xu J, O’Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK. Nature. 2024 May;629(8013):951-956. doi: 10.1038/s41586-024-07327-x. Epub 2024 Apr 17. PMID: 38632403

Development of covalent chemogenetic K2P channel activators. Deal PE, Lee H, Mondal A, Lolicato M, Mendonça PRF, Black H, Jang S, El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL Jr. Cell Chem Biol. 2024 Jul 18;31(7):1305-1323.e9. doi: 10.1016/j.chembiol.2024.06.006. PMID: 39029456

Frequency-multiplexed aberration measurement for confocal microscopy. Pan D, Ge X, Liu YT, Ferger L, Shcherbakova DM, Isacoff E, Ji N. Opt Express. 2024 Jul 29;32(16):28655-28665. doi: 10.1364/OE.525479. PMID: 39538678; PMCID: PMC11595278.

Cell-Specific Optical Control of AMPA Glutamate Receptors with a Photoswitchable Tethered Antagonist. Leippe P, Donthamsetti P, Ko T, Stanley C, Isacoff E, Trauner D. Angew Chem Int Ed Engl. 2024 Dec 2;63(49):e202411181. doi: 10.1002/anie.202411181. Epub 2024 Oct 29. PMID: 39189798.

2023

Homo- and hetero-dimeric subunit interactions set affinity and efficacy in metabotropic glutamate receptors. Habrian C, Latorraca N, Fu Z, Isacoff EY. Nat Commun. 2023 Dec 13;14(1):8288. doi: 10.1038/s41467-023-44013-4. PMID: 38092773 

Ciliary localization of a light-activated neuronal GPCR shapes behavior. Winans AM, Friedmann D, Stanley C, Xiao T, Liu TL, Chang CJ, Isacoff EY. Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2311131120. doi: 10.1073/pnas.2311131120. Epub 2023 Oct 16. PMID: 37844228 

Optical Control of Dopamine D2-like Receptors with Cell-Specific Fast-Relaxing Photoswitches. Hetzler BE, Donthamsetti P, Peitsinis Z, Stanley C, Trauner D, Isacoff EY. J Am Chem Soc. 2023 Aug 30;145(34):18778-18788. doi: 10.1021/jacs.3c02735. Epub 2023 Aug 16. PMID: 37586061

Negative allosteric modulation of the glucagon receptor by RAMP2. Krishna Kumar K, O’Brien ES, Habrian CH, Latorraca NR, Wang H, Tuneew I, Montabana E, Marqusee S, Hilger D, Isacoff EY, Mathiesen JM, Kobilka BK. Cell. 2023 Mar 30;186(7):1465-1477.e18. doi: 10.1016/j.cell.2023.02.028. PMID: 37001505 

Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels. Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG. Nat Struct Mol Biol. 2023 Jun;30(6):841-852. doi: 10.1038/s41594-023-00944-6. Epub 2023 Mar 16. PMID: 36928458

2022

Determinants of synapse diversity revealed by super-resolution quantal transmission and active zone imaging. Newman ZL, Bakshinskaya D, Schultz R, Kenny SJ, Moon S, Aghi K, Stanley C, Marnani N, Li R, Bleier J, Xu K, Isacoff EY. Nat Commun. 2022 Jan 11;13(1):229. doi: 10.1038/s41467-021-27815-2. PMID: 35017509 

2021

Cell specific photoswitchable agonist for reversible control of endogenous dopamine receptors. Donthamsetti P, Winter N, Hoagland A, Stanley C, Visel M, Lammel S, Trauner D, Isacoff E. Nat Commun. 2021 Aug 6;12(1):4775. doi: 10.1038/s41467-021-25003-w. PMID: 34362914; PMCID: PMC8346604.

Conformational rearrangement of the NMDA receptor amino-terminal domain during activation and allosteric modulation. Vyklicky V, Stanley C, Habrian C, Isacoff EY. Nat Commun. 2021 May 11;12(1):2694. doi: 10.1038/s41467-021-23024-z. PMID: 33976221

Selective Photoswitchable Allosteric Agonist of a G Protein-Coupled Receptor. Donthamsetti P, Konrad DB, Hetzler B, Fu Z, Trauner D, Isacoff EY. J Am Chem Soc. 2021 Jun 23;143(24):8951-8956. doi: 10.1021/jacs.1c02586. Epub 2021 Jun 11. PMID: 34115935

In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution. Chen W, Natan RG, Yang Y, Chou SW, Zhang Q, Isacoff EY, Ji N. Nat Commun. 2021 Nov 16;12(1):6630. doi: 10.1038/s41467-021-26965-7. PMID: 34785691 

2020

Experience, circuit dynamics, and forebrain recruitment in larval zebrafish prey capture. Oldfield CS, Grossrubatscher I, Chávez M, Hoagland A, Huth AR, Carroll EC, Prendergast A, Qu T, Gallant JL, Wyart C, Isacoff EY. Elife. 2020 Sep 28;9:e56619. doi: 10.7554/eLife.56619. PMID: 32985972

Dimer interaction in the Hv1 proton channel. Mony L, Stroebel D, Isacoff EY. Proc Natl Acad Sci U S A. 2020 Aug 25;117(34):20898-20907. doi: 10.1073/pnas.2010032117. Epub 2020 Aug 11. PMID: 32788354 

Fast widefield imaging of neuronal structure and function with optical sectioning in vivo. Li Z, Zhang Q, Chou SW, Newman Z, Turcotte R, Natan R, Dai Q, Isacoff EY, Ji N. Sci Adv. 2020 May 8;6(19):eaaz3870. doi: 10.1126/sciadv.aaz3870. eCollection 2020 May. PMID: 32494711

Optical Control of Lysophosphatidic Acid Signaling. Morstein J, Dacheux MA, Norman DD, Shemet A, Donthamsetti PC, Citir M, Frank JA, Schultz C, Isacoff EY, Parrill AL, Tigyi GJ, Trauner D. J Am Chem Soc. 2020 Jun 17;142(24):10612-10616. doi: 10.1021/jacs.0c02154. Epub 2020 Jun 5. PMID: 32469525 

2019

Conformational pathway provides unique sensitivity to a synaptic mGluR. Habrian CH, Levitz J, Vyklicky V, Fu Z, Hoagland A, McCort-Tranchepain I, Acher F, Isacoff EY. Nat Commun. 2019 Dec 5;10(1):5572. doi: 10.1038/s41467-019-13407-8. PMID: 31804469

Genetically Targeted Optical Control of an Endogenous G Protein-Coupled Receptor. Donthamsetti PC, Broichhagen J, Vyklicky V, Stanley C, Fu Z, Visel M, Levitz JL, Javitch JA, Trauner D, Isacoff EY. J Am Chem Soc. 2019 Jul 24;141(29):11522-11530. doi: 10.1021/jacs.9b02895. Epub 2019 Jul 10. PMID: 31291105 

Restoration of high-sensitivity and adapting vision with a cone opsin. Berry MH, Holt A, Salari A, Veit J, Visel M, Levitz J, Aghi K, Gaub BM, Sivyer B, Flannery JG, Isacoff EY. Nat Commun. 2019 Mar 15;10(1):1221. doi: 10.1038/s41467-019-09124-x. PMID: 30874546

Optical control of sphingosine-1-phosphate formation and function. Morstein J, Hill RZ, Novak AJE, Feng S, Norman DD, Donthamsetti PC, Frank JA, Harayama T, Williams BM, Parrill AL, Tigyi GJ, Riezman H, Isacoff EY, Bautista DM, Trauner D. Nat Chem Biol. 2019 Jun;15(6):623-631. doi: 10.1038/s41589-019-0269-7. Epub 2019 Apr 29. PMID: 31036923

2018

Multiplexed temporally focused light shaping for high-resolution multi-cell targeting, Nicolò Accanto, Clément Molinier, Dimitrii Tanese, Emiliano Ronzitti, Zachary L. Newman, Claire Wyart, Ehud Isacoff, Eirini Papagiakoumou, and Valentina Emiliani, Optica 5, 1478-1491 (2018)

Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system. Xiao T, Ackerman CM, Carroll EC, Jia S, Hoagland A, Chan J, Thai B, Liu CS, Isacoff EY, Chang CJ. Nat Chem Biol. 2018 Jul;14(7):655-663. doi: 10.1038/s41589-018-0062-z. Epub 2018 Jun 4. PMID: 29867144 

2017

Input-Specific Plasticity and Homeostasis at the Drosophila Larval Neuromuscular Junction. Newman ZL, Hoagland A, Aghi K, Worden K, Levy SL, Son JH, Lee LP, Isacoff EY. Neuron. 2017 Mar 22;93(6):1388-1404.e10. doi: 10.1016/j.neuron.2017.02.028. Epub 2017 Mar 9. PMID: 28285823

Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor. Berry MH, Holt A, Levitz J, Broichhagen J, Gaub BM, Visel M, Stanley C, Aghi K, Kim YJ, Cao K, Kramer RH, Trauner D, Flannery J, Isacoff EY. Nat Commun. 2017 Nov 30;8(1):1862. doi: 10.1038/s41467-017-01990-7. PMID: 29192252 

Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors. Levitz J, Broichhagen J, Leippe P, Konrad D, Trauner D, Isacoff EY. Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3546-E3554. doi: 10.1073/pnas.1619652114. Epub 2017 Apr 10. PMID: 28396447 

 
Optical Control of Dopamine Receptors Using a Photoswitchable Tethered Inverse Agonist. Donthamsetti PC, Winter N, Schönberger M, Levitz J, Stanley C, Javitch JA, Isacoff EY, Trauner D. J Am Chem Soc. 2017 Dec 27;139(51):18522-18535. doi: 10.1021/jacs.7b07659. Epub 2017 Dec 13. PMID: 29166564 

Sequential Steps of CRAC Channel Activation. Palty R, Fu Z, Isacoff EY. Cell Rep. 2017 May 30;19(9):1929-1939. doi: 10.1016/j.celrep.2017.05.025. PMID: 28564609 

A new mechanism of voltage-dependent gating exposed by KV10.1 channels interrupted between voltage sensor and pore. Tomczak AP, Fernández-Trillo J, Bharill S, Papp F, Panyi G, Stühmer W, Isacoff EY, Pardo LA. J Gen Physiol. 2017 May 1;149(5):577-593. doi: 10.1085/jgp.201611742. Epub 2017 Mar 30. PMID: 28360219 

2016

A Toolkit for Orthogonal and in vivo Optical Manipulation of Ionotropic Glutamate Receptors. Levitz J, Popescu AT, Reiner A, Isacoff EY. Front Mol Neurosci. 2016 Feb 2;9:2. doi: 10.3389/fnmol.2016.00002. eCollection 2016. PMID: 26869877

Mechanism of Assembly and Cooperativity of Homomeric and Heteromeric Metabotropic Glutamate Receptors. Levitz J, Habrian C, Bharill S, Fu Z, Vafabakhsh R, Isacoff EY. Neuron. 2016 Oct 5;92(1):143-159. doi: 10.1016/j.neuron.2016.08.036. Epub 2016 Sep 15. PMID: 27641494

Heterodimerization within the TREK channel subfamily produces a diverse family of highly regulated potassium channels. Levitz J, Royal P, Comoglio Y, Wdziekonski B, Schaub S, Clemens DM, Isacoff EY, Sandoz G. Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):4194-9. doi: 10.1073/pnas.1522459113. Epub 2016 Mar 28. PMID: 27035963

Neuromodulatory Regulation of Behavioral Individuality in Zebrafish. Pantoja C, Hoagland A, Carroll EC, Karalis V, Conner A, Isacoff EY. Neuron. 2016 Aug 3;91(3):587-601. doi: 10.1016/j.neuron.2016.06.016. Epub 2016 Jul 7. PMID: 27397519 

Allosteric substrate switching in a voltage-sensing lipid phosphatase. Grimm SS, Isacoff EY. Nat Chem Biol. 2016 Apr;12(4):261-7. doi: 10.1038/nchembio.2022. Epub 2016 Feb 15. PMID: 26878552

Cooperative Binding of Stromal Interaction Molecule 1 (STIM1) to the N and C Termini of Calcium Release-activated Calcium Modulator 1 (Orai1). Palty R, Isacoff EY. J Biol Chem. 2016 Jan 1;291(1):334-41. doi: 10.1074/jbc.M115.685289. Epub 2015 Nov 6. PMID: 26546674

A family of photoswitchable NMDA receptors. Berlin S, Szobota S, Reiner A, Carroll EC, Kienzler MA, Guyon A, Xiao T, Trauner D, Isacoff EY. Elife. 2016 Mar 1;5:e12040. doi: 10.7554/eLife.12040. PMID: 26929991

Optical control of neuronal activity using a light-operated GIRK channel opener (LOGO). Barber DM, Schönberger M, Burgstaller J, Levitz J, Weaver CD, Isacoff EY, Baier H, Trauner D. Chem Sci. 2016;7(3):2347-2352. doi: 10.1039/C5SC04084A. Epub 2015 Nov 23. PMID: 28090283 

Molecular basis for multimerization in the activation of the epidermal growth factor receptor.Huang Y, Bharill S, Karandur D, Peterson SM, Marita M, Shi X, Kaliszewski MJ, Smith AW, Isacoff EY, Kuriyan J. Elife. 2016 Mar 28;5:e14107. doi: 10.7554/eLife.14107. PMID: 27017828 

A phosphotyrosine switch regulates organic cation transporters. Sprowl JA, Ong SS, Gibson AA, Hu S, Du G, Lin W, Li L, Bharill S, Ness RA, Stecula A, Offer SM, Diasio RB, Nies AT, Schwab M, Cavaletti G, Schlatter E, Ciarimboli G, Schellens JHM,  Isacoff EY, Sali A, Chen T, Baker SD, Sparreboom A, Pabla N. Nat Commun. 2016 Mar 16;7:10880. doi: 10.1038/ncomms10880. PMID: 26979622 

Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins. Chen Y, Bharill S, Altun Z, O’Hagan R, Coblitz B, Isacoff EY, Chalfie M. Mol Biol Cell. 2016 Apr 15;27(8):1272-85. doi: 10.1091/mbc.E15-08-0561. Epub 2016 Mar 3. PMID: 26941331 

MEC-10 and MEC-19 Reduce the Neurotoxicity of the MEC-4(d) DEG/ENaC Channel in Caenorhabditis elegans. Chen Y, Bharill S, O’Hagan R, Isacoff EY, Chalfie M .G3 (Bethesda). 2016 Apr 7;6(4):1121-30. doi: 10.1534/g3.115.023507. PMID: 27172609 

2015

Conformational dynamics of a class C G-protein-coupled receptor. Vafabakhsh R, Levitz J, Isacoff EY. Nature. 2015 Aug 27;524(7566):497-501. doi: 10.1038/nature14679. Epub 2015 Aug 10. PMID: 26258295

 Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, Rockwell NC, Martin SS, Lagarias JC, Isacoff EY. Nat Methods. 2015 Sep;12(9):852-8. doi: 10.1038/nmeth.3480. Epub 2015 Jul 13. PMID: 26167640  

 Orthogonal Optical Control of a G Protein-Coupled Receptor with a SNAP-Tethered Photochromic Ligand. Broichhagen J, Damijonaitis A, Levitz J, Sokol KR, Leippe P, Konrad D, Isacoff EY, Trauner D. ACS Cent Sci. 2015 Oct 28;1(7):383-93. doi: 10.1021/acscentsci.5b00260. Epub 2015 Oct 16. PMID: 27162996 

Subunit composition of a DEG/ENaC mechanosensory channel of Caenorhabditis elegans. Chen Y, Bharill S,  Isacoff EY Chalfie M. Proc Natl Acad Sci U S A. 2015 Sep 15;112(37):11690-5. doi: 10.1073/pnas.1515968112. Epub 2015 Aug 31. PMID: 26324944 

Critical role for Orai1 C-terminal domain and TM4 in CRAC channel gating. Palty R, Stanley C, Isacoff EY. Cell Res. 2015 Aug;25(8):963-80. doi: 10.1038/cr.2015.80. Epub 2015 Jul 3. PMID: 26138675 

Optogenetic Vision Restoration Using Rhodopsin for Enhanced Sensitivity. Gaub BM, Berry MH, Holt AE, Isacoff EY, Flannery JG. Mol Ther. 2015 Oct;23(10):1562-71. doi: 10.1038/mt.2015.121. Epub 2015 Jul 3. PMID: 26137852 

BMP signaling and microtubule organization regulate synaptic strength. Ball RW, Peled ES, Guerrero G, Isacoff EY. Neuroscience. 2015 Apr 16;291:155-66. doi: 10.1016/j.neuroscience.2015.01.069. Epub 2015 Feb 11. PMID: 25681521 

Two-photon brightness of azobenzene photoswitches designed for glutamate receptor optogenetics. Carroll EC, Berlin S, Levitz J, Kienzler MA, Yuan Z, Madsen D, Larsen DS, Isacoff EY. Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):E776-85. doi: 10.1073/pnas.1416942112. Epub 2015 Feb 4. PMID: 25653339 

2014

 Restoration of visual function by expression of a light-gated mammalian ion channel in retinal ganglion cells or ON-bipolar cells. Gaub BM, Berry MH, Holt AE, Reiner A, Kienzler MA, Dolgova N, Nikonov S, Aguirre GD, Beltran WA, Flannery JG, Isacoff EY. Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):E5574-83. doi: 10.1073/pnas.1414162111. Epub 2014 Dec 8. PMID: 25489083 

A spinal opsin controls early neural activity and drives a behavioral light response. Friedmann D, Hoagland A, Berlin S, Isacoff EY. Curr Biol. 2015 Jan 5;25(1):69-74. doi: 10.1016/j.cub.2014.10.055. Epub 2014 Dec 4. PMID: 25484291 

Long wavelength optical control of glutamate receptor ion channels using a tetra-ortho-substituted azobenzene derivative. Rullo A, Reiner A, Reiter A, Trauner D, Isacoff EY, Woolley GA. Chem Commun (Camb). 2014 Dec 4;50(93):14613-5. doi: 10.1039/c4cc06612j. PMID: 25311049

Phospholipase D2 specifically regulates TREK potassium channels via direct interaction and local production of phosphatidic acid. Comoglio Y, Levitz J, Kienzler MA, Lesage F, Isacoff EY, Sandoz G. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13547-52. doi: 10.1073/pnas.1407160111. Epub 2014 Sep 2. PMID: 25197053 

APP homodimers transduce an amyloid-β-mediated increase in release probability at excitatory synapses. Fogel H, Frere S, Segev O, Bharill S, Shapira I, Gazit N, O’Malley T, Slomowitz E, Berdichevsky Y, Walsh DM, Isacoff EY, Hirsch JA, Slutsky I. Cell Rep. 2014 Jun 12;7(5):1560-1576. doi: 10.1016/j.celrep.2014.04.024. Epub 2014 May 15. PMID: 24835997 

Stoichiometry and specific assembly of Best ion channels. Bharill S, Fu Z, Palty R, Isacoff EY. Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6491-6. doi: 10.1073/pnas.1400248111. Epub 2014 Apr 18. PMID: 24748110 

Photoswitching of cell surface receptors using tethered ligands. Reiner A, Isacoff EY. Methods Mol Biol. 2014;1148:45-68. doi: 10.1007/978-1-4939-0470-9_4. PMID: 24718794

Tethered ligands reveal glutamate receptor desensitization depends on subunit occupancy. Reiner A, Isacoff EY. Nat Chem Biol. 2014 Apr;10(4):273-80. doi: 10.1038/nchembio.1458. Epub 2014 Feb 23. PMID: 24561661 

Evoked and spontaneous transmission favored by distinct sets of synapses. Peled ES, Newman ZL, Isacoff EY. Curr Biol. 2014 Mar 3;24(5):484-93. doi: 10.1016/j.cub.2014.01.022. Epub 2014 Feb 20. PMID: 24560571 

2013

Rapid feedback regulation of synaptic efficacy during high-frequency activity at the Drosophila larval neuromuscular junction. Kauwe G, Isacoff EY. Proc Natl Acad Sci U S A. 2013 May 28;110(22):9142-7. doi: 10.1073/pnas.1221314110. Epub 2013 May 14. PMID: 23674684 

AMPA receptor/TARP stoichiometry visualized by single-molecule subunit counting. Hastie P, Ulbrich MH, Wang HL, Arant RJ, Lau AG, Zhang Z, Isacoff EY, Chen L. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5163-8. doi: 10.1073/pnas.1218765110. Epub 2013 Mar 11. PMID: 23479622 

Optical control of metabotropic glutamate receptors. Levitz J, Pantoja C, Gaub B, Janovjak H, Reiner A, Hoagland A, Schoppik D, Kane B, Stawski P, Schier AF, Trauner D, Isacoff EY. Nat Neurosci. 2013 Apr;16(4):507-16. doi: 10.1038/nn.3346. Epub 2013 Mar 3. PMID: 23455609 

2012

Molecular mechanism of the assembly of an acid-sensing receptor ion channel complex. Yu Y, Ulbrich MH, Li MH, Dobbins S, Zhang WK, Tong L, Isacoff EY, Yang J. Nat Commun. 2012;3:1252. doi: 10.1038/ncomms2257. PMID: 23212381

Optical control of endogenous proteins with a photoswitchable conditional subunit reveals a role for TREK1 in GABA(B) signaling. Sandoz G, Levitz J, Kramer RH, Isacoff EY. Neuron. 2012 Jun 21;74(6):1005-14. doi: 10.1016/j.neuron.2012.04.026. PMID: 22726831 

A glutamate switch controls voltage-sensitive phosphatase function. Liu L, Kohout SC, Xu Q, Müller S, Kimberlin CR, Isacoff EY, Minor DL Jr. Nat Struct Mol Biol. 2012 May 6;19(6):633-41. doi: 10.1038/nsmb.2289. PMID: 22562138 

Assembly stoichiometry of the GluK2/GluK5 kainate receptor complex. Reiner A, Arant RJ, Isacoff EY. Cell Rep. 2012 Mar 29;1(3):234-40. doi: 10.1016/j.celrep.2012.01.003. PMID: 22509486 

Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat. Ahrens KF, Heider B, Lee H, Isacoff EY, Siegel RM. Front Neural Circuits. 2012 Mar 22;6:15. doi: 10.3389/fncir.2012.00015. eCollection 2012. PMID: 22461770 

Colloids as mobile substrates for the implantation and integration of differentiated neurons into the mammalian brain. Jgamadze D, Bergen J, Stone D, Jang JH, Schaffer DV, Isacoff EY, Pautot S. PLoS One. 2012;7(1):e30293. doi: 10.1371/journal.pone.0030293. Epub 2012 Jan 25. PMID: 22295079 

Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release. Li D, Hérault K, Isacoff EY, Oheim M, Ropert N. J Physiol. 2012 Feb 15;590(4):855-73. doi: 10.1113/jphysiol.2011.219345. Epub 2012 Jan 4. PMID: 22219341 

Emergence of patterned activity in the developing zebrafish spinal cord. Warp E, Agarwal G, Wyart C, Friedmann D, Oldfield CS, Conner A, Del Bene F, Arrenberg AB, Baier H, Isacoff EY. Curr Biol. 2012 Jan 24;22(2):93-102. doi: 10.1016/j.cub.2011.12.002. Epub 2011 Dec 22. PMID: 22197243 

2011

The pore of the voltage-gated proton channel. Berger TK, Isacoff EY. Neuron. 2011 Dec 22;72(6):991-1000. doi: 10.1016/j.neuron.2011.11.014. PMID: 22196334 

Structural model of the TRPP2/PKD1 C-terminal coiled-coil complex produced by a combined computational and experimental approach. Zhu J, Yu Y, Ulbrich MH, Li MH, Isacoff EY, Honig B, Yang J. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10133-8. doi: 10.1073/pnas.1017669108. Epub 2011 Jun 3. PMID: 21642537 

LiGluR restores visual responses in rodent models of inherited blindness. Caporale N, Kolstad KD, Lee T, Tochitsky I, Dalkara D, Trauner D, Kramer R, Dan Y, Isacoff EY, Flannery JG. Mol Ther. 2011 Jul;19(7):1212-9. doi: 10.1038/mt.2011.103. Epub 2011 May 24. PMID: 21610698

A modern ionotropic glutamate receptor with a K(+) selectivity signature sequence. Janovjak H, Sandoz G, Isacoff EY. Nat Commun. 2011;2:232. doi: 10.1038/ncomms1231. PMID: 21407198

Optical quantal analysis of synaptic transmission in wild-type and rab3-mutant Drosophila motor axons. Peled ES, Isacoff EY. Nat Neurosci. 2011 Apr;14(4):519-26. doi: 10.1038/nn.2767. Epub 2011 Mar 6. PMID: 21378971

Optical probing of a dynamic membrane interaction that regulates the TREK1 channel. Sandoz G, Bell SC, Isacoff EY. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2605-10. doi: 10.1073/pnas.1015788108. Epub 2011 Jan 24. PMID: 21262820

Specializations of a pheromonal glomerulus in the Drosophila olfactory system. Agarwal G, Isacoff E.  J Neurophysiol. 2011 Apr;105(4):1711-21. doi: 10.1152/jn.00591.2010. Epub 2011 Feb 2. PMID: 21289134; PMCID: PMC3075298.

Calmodulin overexpression does not alter Cav1.2 function or oligomerization state. Findeisen, F., Tolia, A., Arant, R., Young Kim, E., Isacoff, E., & Minor, Jr., D. L. (2011). Channels, 5(4), 320–324. https://doi.org/10.4161/chan.5.4.16821

2010

Functional architecture of olfactory ionotropic glutamate receptors. Abuin L, Bargeton B, Ulbrich MH, Isacoff EY, Kellenberger S, Benton R. Neuron. 2011 Jan 13;69(1):44-60. doi: 10.1016/j.neuron.2010.11.042. PMID: 21220098 

Two-photon imaging of calcium in virally transfected striate cortical neurons of behaving monkey. Heider B, Nathanson JL, Isacoff EY, Callaway EM, Siegel RM. PLoS One. 2010 Nov 4;5(11):e13829. doi: 10.1371/journal.pone.0013829. PMID: 21079806

Filtering of visual information in the tectum by an identified neural circuit. Del Bene F, Wyart C, Robles E, Tran A, Looger L, Scott EK, Isacoff EY, Baier H. Science. 2010 Oct 29;330(6004):669-73. doi: 10.1126/science.1192949. PMID: 21030657 

Genetic screen for potassium leaky small mechanosensitive channels (MscS) in Escherichia coli: recognition of cytoplasmic β domain as a new gating element. Koprowski P, Grajkowski W, Isacoff EY, Kubalski A.J Biol Chem. 2011 Jan 7;286(1):877-88. doi: 10.1074/jbc.M110.176131. Epub 2010 Oct 26. PMID: 20978126 

Stoichiometry of the KCNQ1 – KCNE1 ion channel complex. Nakajo K, Ulbrich MH, Kubo Y, Isacoff EY. Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18862-7. doi: 10.1073/pnas.1010354107. Epub 2010 Oct 20. PMID: 20962273 

Multiple C-terminal tail Ca(2+)/CaMs regulate Ca(V)1.2 function but do not mediate channel dimerization. Kim EY, Rumpf CH, Van Petegem F, Arant RJ, Findeisen F, Cooley ES, Isacoff EY, Minor DL Jr. EMBO J. 2010 Dec 1;29(23):3924-38. doi: 10.1038/emboj.2010.260. Epub 2010 Oct 15. PMID: 20953164 

Pseudomonas aeruginosa Homoserine lactone activates store-operated cAMP and cystic fibrosis transmembrane regulator-dependent Cl- secretion by human airway epithelia. Schwarzer C, Wong S, Shi J, Matthes E, Illek B, Ianowski JP, Arant RJ, Isacoff E, Vais H, Foskett JK, Maiellaro I, Hofer AM, Machen TE. J Biol Chem. 2010 Nov 5;285(45):34850-63. doi: 10.1074/jbc.M110.167668. Epub 2010 Aug 25. PMID: 20739289; PMCID: PMC2966100.

Scanless two-photon excitation of channelrhodopsin-2. Papagiakoumou E, Anselmi F, Bègue A, de Sars V, Glückstad J, Isacoff EY, Emiliani V. Nat Methods. 2010 Oct;7(10):848-54. doi: 10.1038/nmeth.1505. Epub 2010 Sep 19. PMID: 20852649 

Alternative splicing of neuroligin regulates the rate of presynaptic differentiation. Lee H, Dean C, Isacoff E. J Neurosci. 2010 Aug 25;30(34):11435-46. doi: 10.1523/JNEUROSCI.2946-10.2010. PMID: 20739565; PMCID: PMC3241922.

A light-gated, potassium-selective glutamate receptor for the optical inhibition of neuronal firing. Janovjak H, Szobota S, Wyart C, Trauner D, Isacoff EY. Nat Neurosci. 2010 Aug;13(8):1027-32. doi: 10.1038/nn.2589. Epub 2010 Jun 27. PMID: 20581843 

Electrochemical coupling in the voltage-dependent phosphatase Ci-VSP. Kohout SC, Bell SC, Liu L, Xu Q, Minor DL Jr, Isacoff EY. Nat Chem Biol. 2010 May;6(5):369-75. doi: 10.1038/nchembio.349. Epub 2010 Apr 4. PMID: 20364128 

The opening of the two pores of the Hv1 voltage-gated proton channel is tuned by cooperativity. Tombola F, Ulbrich MH, Kohout SC, Isacoff EY. Nat Struct Mol Biol. 2010 Jan;17(1):44-50. doi: 10.1038/nsmb.1738. Epub 2009 Dec 20. PMID: 20023640 

2009

Optogenetic dissection of a behavioural module in the vertebrate spinal cord. Wyart C, Del Bene F, Warp E, Scott EK, Trauner D, Baier H, Isacoff EY. Nature. 2009 Sep 17;461(7262):407-10. doi: 10.1038/nature08323. PMID: 19759620 

Structural and molecular basis of the assembly of the TRPP2/PKD1 complex. Yu Y, Ulbrich MH, Li MH, Buraei Z, Chen XZ, Ong AC, Tong L, Isacoff EY, Yang J. Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11558-63. doi: 10.1073/pnas.0903684106. Epub 2009 Jun 25. PMID: 19556541

Molecular recognition and self-assembly special feature: Calix[4]arene-based conical-shaped ligands for voltage-dependent potassium channels. Martos V, Bell SC, Santos E, Isacoff EY, Trauner D, de Mendoza J. Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10482-6. doi: 10.1073/pnas.0813396106. Epub 2009 May 12. PMID: 19435843 

2008

Optical lock-in detection imaging microscopy for contrast-enhanced imaging in living cells. Marriott G, Mao S, Sakata T, Ran J, Jackson DK, Petchprayoon C, Gomez TJ, Warp E, Tulyathan O, Aaron HL, Isacoff EY, Yan Y. Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17789-94. doi: 10.1073/pnas.0808882105. Epub 2008 Nov 12. PMID: 19004775

Rules of engagement for NMDA receptor subunits. Ulbrich MH, Isacoff EY. Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):14163-8. doi: 10.1073/pnas.0802075105. Epub 2008 Sep 8. PMID: 18779583 

Colloid-guided assembly of oriented 3D neuronal networks. Pautot S, Wyart C, Isacoff EY. Nat Methods. 2008 Aug;5(8):735-40. doi: 10.1038/nmeth.1236. Epub 2008 Jul 20. PMID: 18641658

The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor. Tombola F, Ulbrich MH, Isacoff EY. Neuron. 2008 May 22;58(4):546-56. doi: 10.1016/j.neuron.2008.03.026. PMID: 18498736

 Subunit organization and functional transitions in Ci-VSP. Kohout SC, Ulbrich MH, Bell SC, Isacoff EY. Nat Struct Mol Biol. 2008 Jan;15(1):106-8. doi: 10.1038/nsmb1320. Epub 2007 Dec 16. PMID: 18084307

2007

Drosophila huntingtin-interacting protein 14 is a presynaptic protein required for photoreceptor synaptic transmission and expression of the palmitoylated proteins synaptosome-associated protein 25 and cysteine string protein. Stowers RS, Isacoff EY. J Neurosci. 2007 Nov 21;27(47):12874-83. doi: 10.1523/JNEUROSCI.2464-07.2007. PMID: 18032660

 Closing in on the resting state of the Shaker K(+) channel. Pathak MM, Yarov-Yarovoy V, Agarwal G, Roux B, Barth P, Kohout S, Tombola F, Isacoff EY. Neuron. 2007 Oct 4;56(1):124-40. doi: 10.1016/j.neuron.2007.09.023. PMID: 17920020

Mechanisms of photoswitch conjugation and light activation of an ionotropic glutamate receptor. Gorostiza P, Volgraf M, Numano R, Szobota S, Trauner D, Isacoff EY. Proc Natl Acad Sci U S A. 2007 Jun 26;104(26):10865-70. doi: 10.1073/pnas.0701274104. Epub 2007 Jun 19. PMID: 17578923

Remote control of neuronal activity with a light-gated glutamate receptor. Szobota S, Gorostiza P, Del Bene F, Wyart C, Fortin DL, Kolstad KD, Tulyathan O, Volgraf M, Numano R, Aaron HL, Scott EK, Kramer RH, Flannery J, Baier H, Trauner D, Isacoff EY. Neuron. 2007 May 24;54(4):535-45. doi: 10.1016/j.neuron.2007.05.010. PMID: 17521567

Molecular imaging of hydrogen peroxide produced for cell signaling. Miller EW, Tulyathan O, Isacoff EY, Chang CJ. Nat Chem Biol. 2007 May;3(5):263-7. doi: 10.1038/nchembio871. Epub 2007 Apr 1. PMID: 17401379

Subunit counting in membrane-bound proteins. Ulbrich MH, Isacoff EY. Nat Methods. 2007 Apr;4(4):319-21. doi: 10.1038/nmeth1024. Epub 2007 Mar 18. PMID: 17369835

Reversibly caged glutamate: a photochromic agonist of ionotropic glutamate receptors. Volgraf M, Gorostiza P, Szobota S, Helix MR, Isacoff EY, Trauner D. J Am Chem Soc. 2007 Jan 17;129(2):260-1. doi: 10.1021/ja067269o. PMID: 17212390

Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells. Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK. J Neurosci Methods. 2007 Mar 30;161(1):32-8. doi: 10.1016/j.jneumeth.2006.10.005. Epub 2006 Nov 28. PMID: 17126911

All optical interface for parallel, remote, and spatiotemporal control of neuronal activity. Wang S, Szobota S, Wang Y, Volgraf M, Liu Z, Sun C, Trauner D, Isacoff EY, Zhang X. Nano Lett. 2007 Dec;7(12):3859-63. doi: 10.1021/nl072783t. Epub 2007 Nov 23. PMID: 18034506.

2006

The twisted ion-permeation pathway of a resting voltage-sensing domain. Tombola F, Pathak MM, Gorostiza P, Isacoff EY. Nature. 2007 Feb 1;445(7127):546-9. doi: 10.1038/nature05396. Epub 2006 Dec 24. PMID: 17187057

Analysis of a RanGTP-regulated gradient in mitotic somatic cells. Kaláb P, Pralle A, Isacoff EY, Heald R, Weis K. Nature. 2006 Mar 30;440(7084):697-701. doi: 10.1038/nature04589. PMID: 16572176

Molecular handles for the mechanical manipulation of single-membrane proteins in living cells. Gorostiza P, Tombola F, Verdaguer A, Smith SB, Bustamante C, Isacoff EY. IEEE Trans Nanobioscience. 2005 Dec;4(4):269-76. doi: 10.1109/tnb.2005.859552. PMID: 16433292

Allosteric control of an ionotropic glutamate receptor with an optical switch. Volgraf M, Gorostiza P, Numano R, Kramer RH, Isacoff EY, Trauner D. Nat Chem Biol. 2006 Jan;2(1):47-52. doi: 10.1038/nchembio756. Epub 2005 Dec 11. PMID: 16408092

A selective turn-on fluorescent sensor for imaging copper in living cells. Zeng L, Miller EW, Pralle A, Isacoff EY, Chang CJ. J Am Chem Soc. 2006 Jan 11;128(1):10-1. doi: 10.1021/ja055064u. PMID: 16390096 

2005

The cooperative voltage sensor motion that gates a potassium channel. Pathak M, Kurtz L, Tombola F, Isacoff E. J Gen Physiol. 2005 Jan;125(1):57-69. doi: 10.1085/jgp.200409197. PMID: 15623895; PMCID: PMC1414780.

Molecular adhesion of the neuronal synapse reconstituted between live cells and supported lipid bilayer. Pautot, S., Lee, H., Isacoff, E.Y. and Groves, J.T. Nature Chem. Bio. (2005)1, 283-9.

 Voltage-sensing arginines in a potassium channel permeate and occlude cation-selective pores. Tombola F, Pathak MM, Isacoff EY. Neuron. 2005 Feb 3;45(3):379-88. doi: 10.1016/j.neuron.2004.12.047. PMID: 15694325.

Boronate-based fluorescent probes for imaging cellular hydrogen peroxide. Miller EW, Albers AE, Pralle A, Isacoff EY, Chang CJ. J Am Chem Soc. 2005 Nov 30;127(47):16652-9. doi: 10.1021/ja054474f. PMID: 16305254 

Neuronal activation by GPI-linked neuroligin-1 displayed in synthetic lipid bilayer membranes. Baksh MM, Dean C, Pautot S, DeMaria S, Isacoff E, Groves JT. Langmuir. 2005 Nov 8;21(23):10693-8. doi: 10.1021/la051243d. PMID: 16262338; PMCID: PMC1448170.

Heterogeneity in synaptic transmission along a Drosophila larval motor axon. Guerrero G, Reiff DF, Agarwal G, Ball RW, Borst A, Goodman CS, Isacoff EY. Nat Neurosci. 2005 Sep;8(9):1188-96. doi: 10.1038/nn1526. Epub 2005 Aug 14. PMID: 16116446 

In vivo performance of genetically encoded indicators of neural activity in flies. Reiff DF, Ihring A, Guerrero G, Isacoff EY, Joesch M, Nakai J, Borst A. J Neurosci. 2005 May 11;25(19):4766-78. doi: 10.1523/JNEUROSCI.4900-04.2005. PMID: 15888652 

A fluorescent probe designed for studying protein conformational change. Cohen BE, Pralle A, Yao X, Swaminath G, Gandhi CS, Jan YN, Kobilka BK, Isacoff EY, Jan LY. Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):965-70. doi: 10.1073/pnas.0409469102. Epub 2005 Jan 18. PMID: 15657131 

2004

Voltage-sensing arginines in a potassium channel permeate and occlude cation-selective pores. Tombola F, Pathak MM, Isacoff EY. Neuron. 2005 Feb 3;45(3):379-88. doi: 10.1016/j.neuron.2004.12.047. PMID: 15694325 

A selective, cell-permeable optical probe for hydrogen peroxide in living cells. Chang MC, Pralle A, Isacoff EY, Chang CJ. J Am Chem Soc. 2004 Dec 1;126(47):15392-3. doi: 10.1021/ja0441716. PMID: 15563161

Light-activated ion channels for remote control of neuronal firing. Banghart M, Borges K, Isacoff E, Trauner D, Kramer RH. Nat Neurosci. 2004 Dec;7(12):1381-6. doi: 10.1038/nn1356. Epub 2004 Nov 21. PMID: 15558062; PMCID:PMC1447674.

2003

 

The orientation and molecular movement of a k(+) channel voltage-sensing domain. Gandhi CS, Clark E, Loots E, Pralle A, Isacoff EY. Neuron. 2003 Oct 30;40(3):515-25. doi: 10.1016/s0896-6273(03)00646-9. PMID: 14642276 

Protein surface recognition by rational design: nanomolar ligands for potassium channels. Gradl SN, Felix JP, Isacoff EY,   Garcia ML, Trauner D. J Am Chem Soc. 2003 Oct 22;125(42):12668-9. doi: 10.1021/ja036155z. PMID: 14558789 

Oligomeric
Complexes of Neuroligin Mediate Synaptic Adhesion with Neurexin and Induce the Formation of Presynaptic Terminals. Dean, C., Scholl, F.G., Choih, J., Berger, J., Isacoff, E.Y., and Scheiffele, P. Nature Neurosci. 2003 6, 708-16.

 

2002

Tuning FlaSh: redesign of the dynamics, voltage range, and color of the genetically encoded optical sensor of membrane potential. Guerrero G, Siegel MS, Roska B, Loots E, Isacoff EY. Biophys J. 2002 Dec;83(6):3607-18. doi: 10.1016/S0006-3495(02)75361-7. PMID: 12496128 

 

Conformational switch between slow and fast gating modes: allosteric regulation of voltage sensor mobility in the EAG K+ channel. Schönherr R, Mannuzzu LM, Isacoff EY, Heinemann SH. Neuron. 2002 Aug 29;35(5):935-49. doi: 10.1016/s0896-6273(02)00869-3. PMID: 12372287 

 

Structural rearrangements in single ion channels detected optically in living cells. Sonnleitner A, Mannuzzu LM, Terakawa S, Isacoff EY. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12759-64. doi: 10.1073/pnas.192261499. Epub 2002 Sep 12. PMID: 12228726 

2000

Molecular coupling of S4 to a K(+) channel’s slow inactivation gate. Loots E, Isacoff EY. J Gen Physiol. 2000 Nov;116(5):623-36. doi: 10.1085/jgp.116.5.623. PMID: 11055991 

Reconstructing voltage sensor-pore interaction from a fluorescence scan of a voltage-gated K+ channel. Gandhi CS, Loots E, Isacoff EY. Neuron. 2000 Sep;27(3):585-95. doi: 10.1016/s0896-6273(00)00068-4. PMID: 11055440 

Independence and cooperativity in rearrangements of a potassium channel voltage sensor revealed by single subunit fluorescence. Mannuzzu LM, Isacoff EY. J Gen Physiol. 2000 Mar;115(3):257-68. doi: 10.1085/jgp.115.3.257. PMID: 10694254 

1999

Spectroscopic mapping of voltage sensor movement in the Shaker potassium channel. Glauner KS, Mannuzzu LM, Gandhi CS, Isacoff EY. Nature. 1999 Dec 16;402(6763):813-7. doi: 10.1038/45561. PMID: 10617202

 

Functional identification of a goldfish odorant receptor. Speca DJ, Lin DM, Sorensen PW, Isacoff EY, Ngai J, Dittman AH. Neuron. 1999 Jul;23(3):487-98. doi: 10.1016/s0896-6273(00)80802-8. PMID: 10433261 

 

 

Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila. Zito K, Parnas D, Fetter RD, Isacoff EY, Goodman CS. Neuron. 1999 1Apr;22(4):719-29. doi: 10.1016/s0896-6273(00)80731-x. PMID: 10230792 

1998

Protein rearrangements underlying slow inactivation of the Shaker K+ channel. Loots E, Isacoff EY. J Gen Physiol. 1998 Oct;112(4):377-89. doi: 10.1085/jgp.112.4.377. PMID: 9758858 

Three transmembrane conformations and sequence-dependent displacement of the S4 domain in shaker K+ channel gating. Baker OS, Larsson HP, Mannuzzu LM, Isacoff EY. Neuron. 1998 Jun;20(6):1283-94. doi: 10.1016/s0896-6273(00)80507-3. PMID: 9655514 

A permanent ion binding site located between two gates of the Shaker K+ channel. Harris RE, Larsson HP, Isacoff EY. Biophys J. 1998 Apr;74(4):1808-20. doi: 10.1016/s0006-3495(98)77891-9. PMID: 9545043 

1997

Synaptic clustering of Fascilin II and Shaker: essential targeting sequences and role of Dlg. Zito K, Fetter RD, Goodman CS, Isacoff EY. Neuron. 1997 Nov;19(5):1007-16. doi: 10.1016/s0896-6273(00)80393-1. PMID: 9390515 

A genetically encoded optical probe of membrane voltage. Siegel MS, Isacoff EY. Neuron. 1997 Oct;19(4):735-41. doi: 10.1016/s0896-6273(00)80955-1. PMID: 9354320 

1996

Hydrophobic mutations alter the movement of Mg2+ in the pore of voltage-gated potassium channels. Harris RE, Isacoff EY. Biophys J. 1996 Jul;71(1):209-19. doi: 10.1016/S0006-3495(96)79217-2. PMID: 8804604 

Transmembrane movement of the shaker K+ channel S4. Larsson HP, Baker OS, Dhillon DS, Isacoff EY. Neuron. 1996 Feb;16(2):387-97. doi: 10.1016/s0896-6273(00)80056-2. PMID: 8789953 

 

Direct physical measure of conformational rearrangement underlying potassium channel gating. Mannuzzu LM, Moronne MM, Isacoff EY. Science. 1996 Jan 12;271(5246):213-6. doi: 10.1126/science.271.5246.213. PMID: 8539623

1991

Putative receptor for the cytoplasmic inactivation gate in the Shaker K+ channel. Isacoff EY, Jan YN, Jan LY. Nature. 1991 Sep 5;353(6339):86-90. doi: 10.1038/353086a0. PMID: 1881453

1990

Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes. Isacoff EY, Jan YN, Jan LY. Nature. 1990 Jun 7;345(6275):530-4. doi: 10.1038/345530a0. PMID: 2112229

1988

Burst-patterned stimulation promotes nicotinic transmission in isolated perfused rat sympathetic ganglia. Birks RI, Isacoff EY.  J Physiol. 1988 Aug;402:515-32. doi: 10.1113/jphysiol.1988.sp017218. PMID: 2907049 

Conformational pathway provides unique sensitivity to a synaptic mGluR. Habrian CH, Levitz J, Vyklicky V, Fu Z, Hoagland A, McCort-Tranchepain I, Acher F, Isacoff EY. Nat Commun. 2019 Dec 5;10(1):5572. doi: 10.1038/s41467-019-13407-8. PMID: 31804469

Genetically Targeted Optical Control of an Endogenous G Protein-Coupled Receptor. Donthamsetti PC, Broichhagen J, Vyklicky V, Stanley C, Fu Z, Visel M, Levitz JL, Javitch JA, Trauner D, Isacoff EY. J Am Chem Soc. 2019 Jul 24;141(29):11522-11530. doi: 10.1021/jacs.9b02895. Epub 2019 Jul 10. PMID: 31291105 

Restoration of high-sensitivity and adapting vision with a cone opsin. Berry MH, Holt A, Salari A, Veit J, Visel M, Levitz J, Aghi K, Gaub BM, Sivyer B, Flannery JG, Isacoff EY. Nat Commun. 2019 Mar 15;10(1):1221. doi: 10.1038/s41467-019-09124-x. PMID: 30874546

Optical control of sphingosine-1-phosphate formation and function. Morstein J, Hill RZ, Novak AJE, Feng S, Norman DD, Donthamsetti PC, Frank JA, Harayama T, Williams BM, Parrill AL, Tigyi GJ, Riezman H, Isacoff EY, Bautista DM, Trauner D. Nat Chem Biol. 2019 Jun;15(6):623-631. doi: 10.1038/s41589-019-0269-7. Epub 2019 Apr 29. PMID: 31036923

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